Service Code: HS509
Immunization Protocol
| Day 0 | pre-bleed collection, 1st immunization |
| Day 14 | 2nd immunization |
| Day 28 | 3rd immunization |
| Day 38 | test bleed collection |
| Day 42 | 4th immunization |
Development Phases
- Antigen acquisition: receiving antigen provided by the customer
- Phase 1: immunization of 5 balb/c mice, ELISA titer test; more boosters if necessory
- Phase 2: cell fusion, hybridoma selection
- Phase 3: ELISA screening for positive clones, positve clone expansion
- Phase 4: subcloning of interested clones
- Phase 5: cryostorage and production/purification
The monoclonal antibody hybridoma production service includes: immunization of 5 Balb/c mice, one-spleen fusion, and deliver at least one hybridoma line (the clone selected by the customer during the process). As an option, additional clones can be selected/subcloned if requested. In this project, we perform the entire procedure from animal acquisition and immunization to final antibody production of a selected clone. The entire service procedure is outlined below:
To schedule a monoclonal antibody developement project, customer will provide us with at least 3 mg of antigen sample (such as, highly purified protein, peptide-conjugated to KLH, or compound conjugated to a protein carrier).